Author(s): Uthpall Kumar Roy, Md. Kouser, Sangita Chakraborty, Rahima Akter and Abu Bin Ihsan
Abstract: Miliusa velutina (annonaceae) also known as Gandha gajari is an intermediate-sized evergreen medicinal plant reported in the forest of Madhupur and Bhawal of Bangladesh. The present studies aimed to conduct a preliminary phytochemical screening test and to characterize the leaves extract to evaluate analgesic and antidiarrheal activities. To evaluate the analgesic test, tail immersion, and acetic acid-induced writhing methods were employed. On the other hand, the castor oil-induced diarrhea method was used to figure out the antidiarrheal test. The phytochemical screening test affirmed the presence of carbohydrates, steroids, glycosides, flavonoids, phenolic compounds, tannins, and terpenoids in the leaves extract. The crude extract of the plant showed maximum analgesic effect at 300 mg/kg by tail immersion method where the maximum reaction time was recorded at 3.59±0.37 compared with the standard ibuprofen 3.67±0.29. The writhing method also showed the maximum analgesic effect at 200 mg/kg which disclosed 60.37% inhibition. The extract was also tested for the antidiarrheal test which showed mild antidiarrheal effect. To recapitulate, it can be said that the methanolic extract of Miliusa velutina leaves exhibits a potential analgesic activity. However, further study is required to derive the novel bioactive compounds of Miliusa velutina leaves which can play a significant role in different biological activities.